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How ELISA Kits Help Specialists To Diagnose Diseases Quicker And More Efficiently

By Jocelyn Davidson


One cannot help but to stand in awe of the rapid progress made in the medical field. New techniques and medicines are introduced at an astonishing rate. Of course, one of the main fields of research is diagnostics. No one will argue the fact that an early diagnosis ensures a better prognosis and lower treatment costs. ELISA kits, an enzyme linked immunosorbent assay, allow doctors to diagnose a variety of conditions quickly and efficiently.

This test, also known as EIA is a laboratory procedure that is used to measure just how much antibodies and antigens there are in a specific sample of blood. The ability to measure the concentration of antigens and antibodies is a vital part of the diagnostic process. As an added bonus, this test is very easy to administer and many tests can be processed simultaneously.

Antibodies are protein substances that are produced by the body when it detects the presence of dangerous substances. The dangerous substances are called antigens. When antibodies are detected by means of the EIA test, it follows logically that there must be antigens too. The exact identity of the antigens can be determined by identifying the type of antibody in the sample. In this way a diagnosis can be made confidently.

Patients do not experience any form of discomfort during the test. A small sample of blood is removed and then sent to the laboratory. There, a technician will add the sample to a petri dish that is already prepared with antigens. When the antibodies in the blood sample bind with the specific antigen in the petri dish and identification can be made. Further enzymes are then added to see how the antibody and the antigen react to each other.

Diagnosticians can gain a wealth of information from the results of the EIA test. There are a number of different tests, however, each requiring a different kit. The more complex the test the more accurate and detailed the result. A simple test, such as the direct EIA test, produces fast results. The antigens are coated and the antibody will only recognize it if certain enzymes are present as well.

The indirect EIA test saves time and money because it is able to identify both secondary and primary antibodies during the same test cycle. Savings are achieved because the test often negates the need for other tests. In this case the antigens are coated with polystyrene and they can only be detected by the antibodies after two carefully manages stages.

One of the most accurate tests is called the sandwich EIA test. In this test matched pairs of antibodies are utilized. One of the antibodies is coated with polystyrene and the other one is used as an agent for detection. One of the main advantages of this form of the test is that the concentration of analytes can be determined accurately. This can help in diagnosing latent diseases that cannot easily be done with other tests.

Any test or procedure that can help physicians to diagnose a disease at an earlier stage can help save lives. The EIA test certainly enables the accurate and early diagnosis of a variety of conditions. This can help ensure that treatment programs are less invasive and that the prognosis is better.




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